Growth- and Differentiation-related Enzyme Changes in Cytoplasmic Membranes of Acanthamoeba castellanii
نویسندگان
چکیده
منابع مشابه
Plasma and Phagosome Membranes of Acanthamoeba Castellanii
Plasma membranes were isolated from the ameba Acanthamoeba castellanii by low-speed velocity centrifugation followed by equilibrium centrifugation in a sucrose gradient. The isolated membranes had a high ratio of sterol to phospholipid (0.98 moles/mole) and of phospholipid to protein (0.43 mg/mg). The plasma membranes had very low concentrations of DNA, RNA, lipid inositol, and glycerides. Glyc...
متن کاملPhotoinhibition of Growth in Acanthamoeba castellanii Cultures1
Light from 350 to 680 nm at intensities up to 1.62 X 105 ergs per sec per cm2 slowed exponential growth and lowered the maximum yield in axenic cultures of Acanthamoeba castellanii. Photoinhibition was a linear function of light intensity up to 1.25 X 106 ergs per sec per cm2. At higher intensities, growth was too slow to be measured accurately. A photochemical change occurring in the growth me...
متن کاملThe role of proteases in the differentiation of Acanthamoeba castellanii.
Proteases are significant determinants of protozoan pathogenicity and cytolysis of host cells. However, there is now growing evidence of their involvement in cellular differentiation. Acanthamoeba castellanii of the T4 genotype elaborates a number of proteases, which are inhibited by the serine protease inhibitor phenylmethylsulphonyl fluoride. Using this and other selective protease inhibitors...
متن کاملSurvival and growth of Francisella tularensis in Acanthamoeba castellanii.
Francisella tularensis is a highly infectious, facultative intracellular bacterium which causes epidemics of tularemia in both humans and mammals at regular intervals. The natural reservoir of the bacterium is largely unknown, although it has been speculated that protozoa may harbor it. To test this hypothesis, Acanthamoeba castellanii was cocultured with a strain of F. tularensis engineered to...
متن کاملDNA synthesis in growth and encystment of Acanthamoeba castellanii.
Differentiation of Acanthamoeba castellanii into dormant cysts occurs spontaneously in stationary phase cultures, or can be induced experimentally by starvation. Although no further increase in cell density occurred after induction in either case, incorporation of [H]thymidine into DNA continued at a reduced rate through the period when differentiated products (cyst wall components) were formed...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1978
ISSN: 0022-1287
DOI: 10.1099/00221287-107-1-147